CERAMIDE GLYCANASE FROM THE EARTHWORM, LUMBRICUS-TERRESTRIS

被引：16

作者：

CARTER, BZ ^{[1
]}

LI, SC ^{[1
]}

LI, YT ^{[1
]}

机构：

[1] TUFTS UNIV,SCH MED,DEPT BIOCHEM,BOSTON,MA 02111

来源：

BIOCHEMICAL JOURNAL | 1992年 / 285卷

关键词：

D O I：

10.1042/bj2850619

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ceramide glycanase (CGase) is an enzyme that cleaves the linkage between the sugar chain and the ceramide. To make this enzyme readily available, we have developed a simple method for preparing it from the earthworm, Lumbricus terrestris. The method involves Bio-Gel A-0.5m, octyl-Sepharose and p-aminophenylthiogalactoside-agarose column chromatography. By gel filtration, the molecular mass of earthworm CGase was found to be 43.7 kDa. With ganglioside G(M1) as substrate, the optimal pH of this enzyme was found to be between pH 3.5 and 4.0. Earthworm CGase hydrolyses glycolipids only in the presence of a detergent. Among various bile salts tested, sodium cholate was found to be the most effective in stimulating the hydrolysis of G(M1) by this enzyme. Earthworm CGase released intact glycan chains from various glycosphingolipids in which the glycan chain is linked to the ceramide through a beta-glucosyl linkage. It also detached glycan chains from lactosyldialkylglycerol and alkyl-beta-lactosides.

引用

页码：619 / 623

页数：5

共 25 条

[1] HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY AND DENSITOMETRIC DETERMINATION OF BRAIN GANGLIOSIDE COMPOSITIONS OF SEVERAL SPECIES [J].