EXPRESSION OF HIV-1 GP120 AND HUMAN SOLUBLE CD4 BY RECOMBINANT BACULOVIRUSES AND THEIR INTERACTION INVITRO

被引：56

作者：

MORIKAWA, Y

OVERTON, HA

MOORE, JP

WILKINSON, AJ

BRADY, RL

LEWIS, SJ

JONES, IM

机构：

[1] NERC,INST VIROL,MANSFIELD RD,OXFORD OX1 3SR,ENGLAND

[2] CHESTER BEATTY LABS,INST CANC RES,LONDON SW3 6JB,ENGLAND

[3] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1990年 / 6卷 / 06期

关键词：

D O I：

10.1089/aid.1990.6.765

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported. © 1990, Mary Ann Liebert, Inc. All rights reserved.

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页码：765 / 773

页数：9

相关论文

共 25 条

[1] IDENTIFICATION OF THE RESIDUES IN HUMAN CD4 CRITICAL FOR THE BINDING OF HIV [J].

ARTHOS, J ;

DEEN, KC ;

CHAIKIN, MA ;

FORNWALD, JA ;

SATHE, G ;

SATTENTAU, QJ ;

CLAPHAM, PR ;

WEISS, RA ;

MCDOUGAL, JS ;

PIETROPAOLO, C ;

AXEL, R ;

TRUNEH, A ;

MADDON, PJ ;

SWEET, RW .

CELL, 1989, 57 (03) :469-481

[2]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[3] SOLUBLE CD4 BLOCKS THE INFECTIVITY OF DIVERSE STRAINS OF HIV AND SIV FOR T-CELLS AND MONOCYTES BUT NOT FOR BRAIN AND MUSCLE-CELLS [J].

CLAPHAM, PR ;

WEBER, JN ;

WHITBY, D ;

MCINTOSH, K ;

DALGLEISH, AG ;

MADDON, PJ ;

DEEN, KC ;

SWEET, RW ;

WEISS, RA .

NATURE, 1989, 337 (6205) :368-370

[4] THE CD4 (T4) ANTIGEN IS AN ESSENTIAL COMPONENT OF THE RECEPTOR FOR THE AIDS RETROVIRUS [J].

DALGLEISH, AG ;

BEVERLEY, PCL ;

CLAPHAM, PR ;

CRAWFORD, DH ;

GREAVES, MF ;

WEISS, RA .

NATURE, 1984, 312 (5996) :763-767

[5] A SOLUBLE FORM OF CD4 (T4) PROTEIN INHIBITS AIDS VIRUS-INFECTION [J].

DEEN, KC ;

MCDOUGAL, JS ;

INACKER, R ;

FOLENAWASSERMAN, G ;

ARTHOS, J ;

ROSENBERG, J ;

MADDON, PJ ;

AXEL, R ;

SWEET, RW .

NATURE, 1988, 331 (6151) :82-84

[6] MODEL FOR INTRACELLULAR FOLDING OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 GP120 [J].

FENNIE, C ;

LASKY, LA .

JOURNAL OF VIROLOGY, 1989, 63 (02) :639-646

[7] A SOLUBLE CD4 PROTEIN SELECTIVELY INHIBITS HIV REPLICATION AND SYNCYTIUM FORMATION [J].

HUSSEY, RE ;

RICHARDSON, NE ;

KOWALSKI, M ;

BROWN, NR ;

CHANG, HC ;

SILICIANO, RF ;

DORFMAN, T ;

WALKER, B ;

SODROSKI, J ;

REINHERZ, EL .

NATURE, 1988, 331 (6151) :78-81

[8] LYMPHOCYTE-T T4 MOLECULE BEHAVES AS THE RECEPTOR FOR HUMAN RETROVIRUS LAV [J].

KLATZMANN, D ;

CHAMPAGNE, E ;

CHAMARET, S ;

GRUEST, J ;

GUETARD, D ;

HERCEND, T ;

GLUCKMAN, JC ;

MONTAGNIER, L .

NATURE, 1984, 312 (5996) :767-768

[9] DELINEATION OF A REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP120 GLYCOPROTEIN CRITICAL FOR INTERACTION WITH THE CD4 RECEPTOR [J].

LASKY, LA ;

NAKAMURA, G ;

SMITH, DH ;

FENNIE, C ;

SHIMASAKI, C ;

PATZER, E ;

BERMAN, P ;

GREGORY, T ;

CAPON, DJ .

CELL, 1987, 50 (06) :975-985

[10] CORRECTED CD4 SEQUENCE [J].

LITTMAN, DR ;

MADDON, PJ ;

AXEL, R .

CELL, 1988, 55 (04) :541-541