Depth of penetration;
Enterococcus faecalis;
Methylene blue;
Photodynamic therapy;
D O I:
10.7860/JCDR/2014/11047.5155
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
Context: The success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. Conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. Newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. Aim & Objectives: The basic aim of this study was assessment of the antimicrobial efficacy of Photodynamic Therapy in deeper dentinal tubules for effective disinfection of root canals using microbiological and scanning electron microscopic examination in vitro. Materials and Methods: The study was conducted at Teerthanker Mahaveer Dental College & Research Centre. The teeth required for study was collected from Department of Oral and Maxillofacial Surgery. Only freshly extracted 20 intact, non carious single rooted teeth which were indicated for orthodontic treatment were taken for this study. Statistical analysis was done using Student's Unpaired t -test were at (p<0.001) was found to be highly significant. Microbiological examination of samples were done and colony forming units were counted to assess the disinfection potential of photodynamic therapy. Scanning electron microscopic examination of samples was done to check penetration of bacteria's into deeper dentinal tubules. Results: On examination, there was a marked reduction in microbial growth after use of photodynamic therapy. On scanning electron microscopic examination, it was observed that there were less number of bacteria's in deeper dentinal tubules in case of PDT group as compared to control group. Conclusion: The results of the present study indicate that PDT can be effectively used during antimicrobial procedures along with conventional disinfection procedure for sterilization of root canals.
机构:
CETAO, Sao Paulo, BrazilCtr Pesquisa & Posgrad Sao Leopoldo Mandic, Campinas, SP, Brazil
Nunez, Silvia C.
;
Hamblim, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USACtr Pesquisa & Posgrad Sao Leopoldo Mandic, Campinas, SP, Brazil
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Garcez, Aguinaldo Silva
;
Nunez, Silvia Cristina
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Nunez, Silvia Cristina
;
Hamblin, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USACtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Hamblin, Michael R.
;
Ribeiro, Martha Simoes
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
机构:
King Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi ArabiaKing Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi Arabia
Javed, Fawad
;
Romanos, Georgios E.
论文数: 0引用数: 0
h-index: 0
机构:
SUNY Stony Brook, Sch Dent Med, Dept Dent Med, Stony Brook, NY 11794 USAKing Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi Arabia
机构:
CETAO, Sao Paulo, BrazilCtr Pesquisa & Posgrad Sao Leopoldo Mandic, Campinas, SP, Brazil
Nunez, Silvia C.
;
Hamblim, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USACtr Pesquisa & Posgrad Sao Leopoldo Mandic, Campinas, SP, Brazil
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Garcez, Aguinaldo Silva
;
Nunez, Silvia Cristina
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Nunez, Silvia Cristina
;
Hamblin, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 USA
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USACtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
Hamblin, Michael R.
;
Ribeiro, Martha Simoes
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, BrazilCtr Laser & Aplicacoes IPEN CNEN SP, BR-05508000 Sao Paulo, Brazil
机构:
King Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi ArabiaKing Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi Arabia
Javed, Fawad
;
Romanos, Georgios E.
论文数: 0引用数: 0
h-index: 0
机构:
SUNY Stony Brook, Sch Dent Med, Dept Dent Med, Stony Brook, NY 11794 USAKing Saud Univ, Coll Appl Med Sci, Imaging & Biomechan Lab 3D, Eng AB Res Chair Growth Factors & Bone Regenerat, Riyadh, Saudi Arabia