LOCALIZATION AND FUNCTIONS OF JAPANESE ENCEPHALITIS-VIRUS NONSTRUCTURAL PROTEINS NS3 AND NS5 FOR VIRAL-RNA SYNTHESIS IN THE INFECTED-CELLS

被引：30
作者：
EDWARD, Z
TAKEGAMI, T
机构：
[1] KANAZAWA MED UNIV,MED RES INST,DIV TROP MED,1-1 DAIGAKU,UCHINADA,ISHIKAWA 92002,JAPAN
[2] KANAZAWA MED UNIV,DEPT MICROBIOL,ISHIKAWA 92002,JAPAN
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1993年 / 37卷 / 03期
关键词：
JAPANESE ENCEPHALITIS VIRUS; NONSTRUCTURAL PROTEINS NS3 AND NS5; RNA REPLICATION;
D O I：
10.1111/j.1348-0421.1993.tb03206.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Recently it has been reported that Japanese encephalitis virus (JEV)-specific RNAs can be synthesized in vitro in the subcellular fraction including outer-nuclear membrane (Takegami and Hotta, 1989). The results of Western blot analysis and indirect immunofluorescence test using two kinds of monospecific antisera against JEV nonstructural proteins NS3 and NS5 showed that NS3 and NS5 were membrane-associated proteins and formed the complex at the perinuclear site in the infected cells. Both antisera against NS3 and NS5 inhibited in vitro RNA synthesis. These results suggest that NS5 and NS3 play important role(s) in flavivirus RNA replication.
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页码：239 / 243
页数：5
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