MICROSOMAL IMMOBILIZED-ENZYME-REACTOR FOR ONLINE PRODUCTION OF GLUCURONIDES IN A HPLC COLUMN

被引：18

作者：

ALEBICKOLBAH, T ^{[1
]}

WAINER, IW ^{[1
]}

机构：

[1] MCGILL UNIV,MONTREAL GEN HOSP,DEPT ONCOL,DIV PHARMACOKINET,1650 CEDAR AVE,RMB 7113,MONTREAL H3G 1A4,QUEBEC,CANADA

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 11-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; IMMOBILIZED-ARTIFICIAL-MEMBRANE (IAM) SUPPORT; MICROSOMAL IMMOBILIZED ENZYME REACTOR (IMER); GLUCURONIDATION;

D O I：

10.1007/BF02274110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonsolubilized rat liver microsomes have been noncovalently immobilized on an immobilized artifical membrane (IAM) HPLC chromatographic support to create a microsomal immobilized enzyme reactor (MIC-IMER). The MIC-IMER has been used for the on-line synthesis and analysis of Phase II metabolism conjugates. The activity of the MIC-IMER was investigated by following the in vitro glucuronidation of two well known UDP-glucuronosyltransferase (UDPGT; EC 2.4.1.17) substrates, 4-nitrophenol (4NP) and 4-methylumbelliferone (4Me7OHC). The substrates underwent conjugation with UDPGA and the rate of conjugation reaction in the MIC-IMER was compared, with the same reaction in the test-tube.

引用

页码：608 / 612

页数：5

共 10 条

[1] ENZYME-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY STATIONARY PHASES AS METABOLIC REACTORS - IMMOBILIZATION OF NON-SOLUBILIZED RAT-LIVER MICROSOMES ON AN IMMOBILIZED ARTIFICIAL MEMBRANE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SUPPORT [J].