MICROSOMAL IMMOBILIZED-ENZYME-REACTOR FOR ONLINE PRODUCTION OF GLUCURONIDES IN A HPLC COLUMN

被引：18
作者：
ALEBICKOLBAH, T [1 ]
WAINER, IW [1 ]
机构：
[1] MCGILL UNIV,MONTREAL GEN HOSP,DEPT ONCOL,DIV PHARMACOKINET,1650 CEDAR AVE,RMB 7113,MONTREAL H3G 1A4,QUEBEC,CANADA
来源：
CHROMATOGRAPHIA | 1993年 / 37卷 / 11-12期
关键词：
COLUMN LIQUID CHROMATOGRAPHY; IMMOBILIZED-ARTIFICIAL-MEMBRANE (IAM) SUPPORT; MICROSOMAL IMMOBILIZED ENZYME REACTOR (IMER); GLUCURONIDATION;
D O I：
10.1007/BF02274110
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Nonsolubilized rat liver microsomes have been noncovalently immobilized on an immobilized artifical membrane (IAM) HPLC chromatographic support to create a microsomal immobilized enzyme reactor (MIC-IMER). The MIC-IMER has been used for the on-line synthesis and analysis of Phase II metabolism conjugates. The activity of the MIC-IMER was investigated by following the in vitro glucuronidation of two well known UDP-glucuronosyltransferase (UDPGT; EC 2.4.1.17) substrates, 4-nitrophenol (4NP) and 4-methylumbelliferone (4Me7OHC). The substrates underwent conjugation with UDPGA and the rate of conjugation reaction in the MIC-IMER was compared, with the same reaction in the test-tube.
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页码：608 / 612
页数：5
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