MONOCLONAL-ANTIBODIES TO PORCINE TUMOR-NECROSIS-FACTOR-ALPHA - DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：10
作者：
SU, XZ
PRESTWOOD, AK
OTTO, CM
PRICKETT, D
MCGRAW, RA
机构：
[1] UNIV GEORGIA,COLL VET MED,DEPT SMALL ANIM MED,ATHENS,GA 30602
[2] UNIV GEORGIA,COLL VET MED,DEPT PARASITOL,ATHENS,GA 30602
来源：
JOURNAL OF IMMUNOASSAY | 1992年 / 13卷 / 02期
关键词：
PORCINE TUMOR NECROSIS FACTOR-ALPHA; MONOCLONAL ANTIBODIES; ENZYME-LINKED IMMUNOSORBENT ASSAY;
D O I：
10.1080/15321819208021230
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Five hybridomas (4F4, 14H1, 9B4, 6E10, and 8G7) secreting antibodies to porcine tumor necrosis factor alpha (PTNF-alpha) were obtained from one fusion. Four of the 5 monoclonal antibodies (Mab) recognized recombinant PTNF-alpha (rPTNF-alpha) on western blot and were able to neutralize both rPTNF-alpha and native (released by porcine macrophages) PTNF (nPTNF-alpha, only 4F4, 14H1, and 9B4 tested for the neutralization of nPTNF-alpha) in vitro. A sandwich enzyme-linked immunosorbent assay (ELISA) for PTNF-alpha was developed using Mab 4F4 and purified rabbit polyclonal antibodies against PTNF-alpha. The test detected PTNF-alpha concentrations as low as 400 pg/ml and did not cross react with native porcine TNF-beta, recombinant human TNF-alpha, recombinant mouse TNF-beta or native mouse TNF-alpha. The Mabs and the ELISA should be useful for assessing PTNF-alpha levels in swine serum during disease processes and possibly for alleviation of toxic effects of TNF-alpha.
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收藏
页码：253 / 272
页数：20
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