CONTENT OF FREE-CHOLESTEROL AND CHOLESTEROL ESTERS IN THE SERUM OF RATS AFTER A DIET CONTAINING OIL WITH AND WITHOUT CHOLESTEROL

This study was designed to investigate the influence of a high cholesterol diet, compared with an oil containing diet without cholesterol, on the state of esterification of cholesterol in rat serum. The following cholesterol containing fractions were separated by thin-layer chromatography, nonesterified cholesterol and the cholesterol esters-cholesterol palmitate (CHP), cholesterol oleate (CHO), cholesterol linoleate (CHL), cholesterol arachidonate (CHA) and cholesterol esters of other long chain fatty acids with C20 - C22 (carbon atoms) (CHC). All these esters were isolated after a normal diet as well as after the cholesterol diet and the oil containing diet without cholesterol. The high cholesterol diet intensified synthesis of all cholesterol esters, while the nonesterified fraction was severely decreased on prolonging the diet. The diet containing oil diet without cholesterol esterification. The esterification with staturated fatty acids, was more intensive but only after a prolonged period of on the diet.