CHROMIUM OXALATE - NEW SPIN LABEL BROADENING AGENT FOR USE WITH THYLAKOIDS

被引:73
作者
BERG, SP
NESBITT, DM
机构
[1] Department of Biological Sciences, University of Denver, Denver
关键词
Chloroplast; Chromium oxalate; Electron transport; ESR; Photophosphorylation; Spin label broadening; Tempamine; Thylakoid;
D O I
10.1016/0005-2728(79)90068-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Potassium tris(oxalato)chromate(III) trihydrate (chromium oxalate) has been shown to be a more useful broadening agent than potassium ferricyanide for the spin label 2,2,6,6-tetramethylpiperidine-N-oxy-4-amine (Tempamine) in thylakoid suspensions. Our data show that chromium oxalate is less permeable than ferricyanide, does not inhibit thylakoid electron transport or photophosphorylation, and is not photoreduced by thylakoids. © 1979.
引用
收藏
页码:608 / 615
页数:8
相关论文
共 24 条