CYCLOHEXIMIDE-RESISTANCE IN CHINESE-HAMSTER OVARY CELLS AND HUMAN FIBROBLAST CELLS - CYTOGENETIC AND BIOCHEMICAL CHARACTERIZATION

被引：0

作者：

POCHE, H

NIERHAUS, KH

ZAKRZEWSKI, S

机构：

[1] MAX PLANCK INST MOLEC GENET,WITTMANN ABT,D-1000 BERLIN 33,FED REP GER

[2] FREE UNIV BERLIN,INST HUMAN GENET,D-1000 BERLIN 19,FED REP GER

来源：

HUMAN GENETICS | 1979年 / 50卷 / 03期

关键词：

D O I：

10.1007/BF00399396

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cycloheximide(CHM)-resistant mutant Chinese hamster ovary (CHO) and human cells were induced with N-nitrosomethylurea (NMU) and ethyl methanesulfonate (EMS); the mutants were viable and showed unlimited growth in the presence of CHM (7×10-7M), whereas this concentration inhibits protein synthesis in vivo as well as in vitro. No numerical or structural chromosomal aberrations were found in the mutant cells. In vitro analysis shows that the ribosomes confer resistance against cycloheximide. © 1979 Springer-Verlag.

引用

页码：297 / 305

页数：9

共 24 条

[1] SOMATIC-CELL MUTATION - DETECTION AND QUANTIFICATION OF X-RAY-INDUCED MUTATION IN CULTURED, DIPLOID HUMAN FIBROBLASTS [J].