SOMATIC HYBRIDS OF SUNFLOWER (HELIANTHUS-ANNUUS L) IDENTIFIED AT THE CALLUS STAGE BY ISOENZYME ANALYSIS

被引：6

作者：

BARTH, S ^{[1
]}

VOESTE, D ^{[1
]}

SCHNABL, H ^{[1
]}

机构：

[1] UNIV BONN, INST LANDWIRTSCHAFTL BOT, MECKENHEIMER ALLEE 176, W-5300 BONN 1, GERMANY

来源：

BOTANICA ACTA | 1993年 / 106卷 / 01期

关键词：

SUNFLOWER; ELECTROFUSION; PROTOPLAST; ISOENZYME; REGENERATION;

D O I：

10.1111/j.1438-8677.1993.tb00343.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dark grown hypocotyl protoplasts from Helianthus annuus L. cvs. Cerflor and Euroflor were electrically fused to produce somatic hybrids. Following fusion, the protoplasts were cultivated in agarose droplets for four weeks. Macroscopic visible calli (0.1-0.3 mm) were transferred onto solid medium and calli reaching a size of 3 mm were collected. Their iso-enzyme patterns were analysed based on two different isoenzymes that allow discrimination between the two cultivars used for fusion. From the examined calli, about 26% showed an isoenzyme pattern of putative binary heterokaryocytes. The isoenzyme pattern of the nonfused control revealed less than 5% of possibly chimeric colonies.

引用

页码：100 / 102

页数：3

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