Purification and characterization of a keratinolytic protease produced by probiotic Bacillus subtilis

被引:30
作者
Grohs Ferrareze, Patricia Aline [1 ]
Folmer Correa, Ana Paula [1 ]
Brandelli, Adriano [1 ]
机构
[1] Univ Fed Rio Grande do Sul, Lab Bioquim & Microbiol Aplicada, Inst Ciencia & Tecnol Alimentos, BR-91501970 Porto Alegre, RS, Brazil
关键词
Biodegradation; Feather keratin; Hard proteins; Keratinase;
D O I
10.1016/j.bcab.2016.05.009
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbial biodegradation of waste feathers constitute an alternative to convert hard proteins into valuable products through non-polluting processes. The probiotic Bacillus subtilis FTC02PR1 demonstrated efficiency for degradation of feather meal, white feathers and melanized feathers. Only a few reports describe the microbial degradation of highly resistant melanized feathers. Human hair, a source of pure alpha-keratin, was poorly degraded. A keratinolytic protease produced by this strain was purified by liquid chromatography on Sephadex G-75 and DEAE Sepharose. The enzyme was characterized as a serine proteinase with approximately 30 kDa, and optimal activity at 60 degrees C and under a broad pH range 6.0-11.0). Thermal tolerance at 55 degrees C was significantly increased in the presence of manganese ions. This enzyme may be useful for hydrolysis of recalcitrant proteins. The production of keratinases by probiotic bacterial strains may concomitantly allow better use of keratin-based feeds, due to the possibility of diet supplementation with beneficial microorganisms. (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码:102 / 109
页数:8
相关论文
共 54 条
[1]   Secretion of keratinolytic enzymes and keratinolysis by Scopulariopsis brevicaulis and Trichophyton mentagrophytes:: regression analysis [J].
Anbu, P. ;
Gopinath, S. C. B. ;
Hilda, A. ;
Mathivanan, N. ;
Annadurai, G. .
CANADIAN JOURNAL OF MICROBIOLOGY, 2006, 52 (11) :1060-1069
[2]   Protein precipitation and denaturation by dimethyl sulfoxide [J].
Arakawa, Tsutomu ;
Kita, Yoshiko ;
Timasheff, Serge N. .
BIOPHYSICAL CHEMISTRY, 2007, 131 (1-3) :62-70
[3]   Substrate specifcity of alkaline protease from alkaliphilic feather-degrading Nesterenkonia sp AL20 [J].
Bakhtiar, S ;
Estiveira, RJ ;
Hatti-Kaul, R .
ENZYME AND MICROBIAL TECHNOLOGY, 2005, 37 (05) :534-540
[4]   Purification and characterization of an extracellular keratinase from a hornmeal-degrading Bacillus subtilis MTCC (9102) [J].
Balaji, S. ;
Kumar, M. Senthil ;
Karthikeyan, R. ;
Kumar, Ramadhar ;
Kirubanandan, S. ;
Sridhar, R. ;
Sehgal, P. K. .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24 (11) :2741-2745
[5]   Selective DMSO-induced conformational changes in proteins from Raman optical activity [J].
Batista, Andrea N. L. ;
Batista, Joao M., Jr. ;
Bolzani, Vanderlan S. ;
Furlan, Maysa ;
Blanch, Ewan W. .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2013, 15 (46) :20147-20152
[6]   Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond [J].
Brandelli, Adriano .
FOOD AND BIOPROCESS TECHNOLOGY, 2008, 1 (02) :105-116
[7]   Microbial enzymes for bioconversion of poultry waste into added-value products [J].
Brandelli, Adriano ;
Sala, Luisa ;
Kalil, Susana Juliano .
FOOD RESEARCH INTERNATIONAL, 2015, 73 :3-12
[8]   Biochemical features of microbial keratinases and their production and applications [J].
Brandelli, Adriano ;
Daroit, Daniel J. ;
Riffel, Alessandro .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (06) :1735-1750
[9]  
Bressollier P, 1999, APPL ENVIRON MICROB, V65, P2570
[10]   Colourful parrot feathers resist bacterial degradation [J].
Burtt, Edward H., Jr. ;
Schroeder, Max R. ;
Smith, Lauren A. ;
Sroka, Jenna E. ;
McGraw, Kevin J. .
BIOLOGY LETTERS, 2011, 7 (02) :214-216