DEVELOPMENT OF AN INSTRUMENTAL CONFIGURATION FOR PSEUDO-ELECTROCHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY

被引：37

作者：

DEKKERS, SEG ^{[1
]}

TJADEN, UR ^{[1
]}

VANDERGREEF, J ^{[1
]}

机构：

[1] LEIDEN UNIV, LEIDEN AMSTERDAM CTR DRUG RES, DIV ANALYT CHEM, 2300 RA LEIDEN, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 712卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00508-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of an instrumental configuration for the application of the electric field in pseudo-electrochromatography is described. To increase detection sensitivity and selectivity, special attention has been paid to the hyphenation of PEC with electrospray ionization mass spectrometry. Problems encountered in previous instrumental configurations like formation of gas bubbles have been overcome by using liquid junctions to apply the electric field over the column. Some sulfonamides and a nonapeptide have been used as test compounds to compare pseudo-electrochromatography with micro-liquid chromatography. The characteristic features of pseudo-electrochromatography on retention behaviour and separation efficiency are demonstrated.

引用

页码：201 / 209

页数：9

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