INTEGRATION OF DNA FRAGMENTS BY ILLEGITIMATE RECOMBINATION IN SACCHAROMYCES-CEREVISIAE

被引：275

作者：

SCHIESTL, RH

PETES, TD

机构：

[1] Department of Biology, University of North Carolina, Chapel Hill

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 17期

关键词：

YEAST; NONHOMOLOGOUS RECOMBINATION; RESTRICTION ENZYMES;

D O I：

10.1073/pnas.88.17.7585

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA fragments (generated by BamHI treatment) with no homology to the yeast genome were transformed into Saccharomyces cerevisiae. When the fragments were transformed in the presence of the BamHI enzyme, they integrated into genomic BamHI sites. When the fragments were transformed in the absence of the enzyme, they integrated into genomic G-A-T-C sites. Since the G-A-T-C sequence is present at the ends of BamHI fragments, this result indicates that four base pairs of homology are sufficient for some types of mitotic recombination.

引用

页码：7585 / 7589

页数：5

共 38 条

[1] MITOTIC GENE CONVERSION LENGTHS, CO-CONVERSION PATTERNS, AND THE INCIDENCE OF RECIPROCAL RECOMBINATION IN A SACCHAROMYCES-CEREVISIAE PLASMID SYSTEM [J].