DEOXYRIBONUCLEOLYTIC ACTIVITY OF ALPHA-MOMORCHARIN AND BETA-MOMORCHARIN

被引：31

作者：

GO, TTM ^{[1
]}

YEUNG, HW ^{[1
]}

FONG, WP ^{[1
]}

机构：

[1] CHINESE UNIV HONG KONG, DEPT BIOCHEM, SHA TIN, HONG KONG

来源：

LIFE SCIENCES | 1992年 / 51卷 / 17期

关键词：

D O I：

10.1016/0024-3205(92)90634-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Alpha- and beta-momorcharins were purified by an improved procedure using the affinity Affi-gel Blue gel and the ion exchange Mono-S FPLC column. Both purified alpha- and beta-momorcharins possessed deoxyribonucleolytic activity. Under normal digestion conditions, they cleaved the supercoiled, double-stranded SV-40 DNA to produce nicked circular and linear DNAs. Prolonged incubation did not have any further effects. On the other hand, the linear DNAs. lambda, Ad-2 and T7 were not digested by alpha- nor beta-momorcharin. Thus, it appears that the conformation of the DNA may be the determining factor for the deoxyribonucleolytic activity of these momorcharins.

引用

页码：1347 / 1353

页数：7

共 24 条

[1] TRICHOKIRIN, A RIBOSOME-INACTIVATING PROTEIN FROM THE SEEDS OF TRICHOSANTHES-KIRILOWII MAXIMOWICZ - PURIFICATION, PARTIAL CHARACTERIZATION AND USE FOR PREPARATION OF IMMUNOTOXINS [J].

CASELLAS, P ;

DUSSOSSOY, D ;

FALASCA, AI ;

BARBIERI, L ;

GUILLEMOT, JC ;

FERRARA, P ;

BOLOGNESI, A ;

CENINI, P ;

STIRPE, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (03) :581-588

[2] THE TERMINATION OF EARLY-PREGNANCY IN THE MOUSE BY BETA-MOMORCHARIN [J].

CHAN, WY ;

TAM, PPL ;

YEUNG, HW .

CONTRACEPTION, 1984, 29 (01) :91-100

[3] 2ND CYTO-TOXIC PATHWAY OF DIPHTHERIA-TOXIN SUGGESTED BY NUCLEASE ACTIVITY [J].

CHANG, MP ;

BALDWIN, RL ;

BRUCE, C ;

WISNIESKI, BJ .

SCIENCE, 1989, 246 (4934) :1165-1168

[4]

ENDO Y, 1983, J BIOL CHEM, V258, P2662

[5] ENZYMATIC-PROPERTIES OF RIBOSOME-INACTIVATING PROTEINS (RIPS) AND RELATED TOXINS [J].

FONG, WP ;

WONG, RNS ;

GO, TTM ;

YEUNG, HW .

LIFE SCIENCES, 1991, 49 (25) :1859-1869

[6] PURIFICATION OF IMMUNOTOXINS CONTAINING RICIN-A-CHAIN AND ABRIN-A-CHAIN USING BLUE SEPHAROSE CL-6B [J].

KNOWLES, PP ;

THORPE, PE .

ANALYTICAL BIOCHEMISTRY, 1987, 160 (02) :440-443

[7] CONFORMATION OF ABORTIFACIENT PROTEINS - TRICHOSANTHIN, ALPHA-MOMORCHARIN AND BETA-MOMORCHARIN [J].

KUBOTA, S ;

YEUNG, HW ;

YANG, JT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 871 (01) :101-106

[8] MATURATION OF HEAD OF BACTERIOPHAGE-T4 .1. DNA PACKAGING EVENTS [J].

LAEMMLI, UK ;

FAVRE, M .

JOURNAL OF MOLECULAR BIOLOGY, 1973, 80 (04) :575-599

[9]

LAW LK, 1983, J REPROD FERTIL, V69, P597

[10] THE IMMUNOSUPPRESSIVE ACTIVITIES OF 2 ABORTIFACIENT PROTEINS ISOLATED FROM THE SEEDS OF BITTER-MELON (MOMORDICA-CHARANTIA) [J].

LEUNG, SO ;

YEUNG, HW ;

LEUNG, KN .

IMMUNOPHARMACOLOGY, 1987, 13 (03) :159-171

← 1 2 3 →