IDENTIFICATION OF 3',5'-CYCLIC-AMP IN AXENIC CULTURES OF LEMNA-PAUCICOSTATA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14

作者：

GANGWANI, L ^{[1
]}

TAMOT, BK ^{[1
]}

KHURANA, JP ^{[1
]}

MAHESHWARI, SC ^{[1
]}

机构：

[1] TRIBHUVAN UNIV,CENT DEPT BOT,KATMANDU,NEPAL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91007-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotides were purified from the extract of aseptically grown Lemna paucicostata 6746 by low-pressure column chromatography and thin-layer chromatography. In this partially purified sample, a compound has been identified, using a highly sensitive HPLC-fluorometric detection method, with chromatographic properties and substrate specificity for cyclic nucleotide phosphodiesterase similar to authentic 3′,5′-cyclic AMP. The level of cAMP has been estimated to be 70-80 pmol/g fresh wt. © 1991.

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页码：1113 / 1119

页数：7

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