SEROTONIN RECEPTORS EXPRESSED IN XENOPUS-OOCYTES BY MESSENGER-RNA FROM BRAIN MEDIATE A CLOSING OF K+ MEMBRANE CHANNELS

Membrane currents evoked by serotonin (5-HT) were studied in Xenopus oocytes injected with rat brain mRNA. Intracellular EGTA was used to abolish the Ca2+-dependent oscillatory Cl- current to 5-HT, revealing an underlying smooth inward current. This was associated with a decreased membrane conductance, was antagonized by Ba2+ and Zn2+ (but not TEA), and probably arises through a closing of K+ channels. Half-maximal responses were obtained with 30 nM 5-HT, while 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT) was ineffective. Furthermore, methysergide, mianserin and lysergic acid antagonized the K+-closing response to 5-HT, consistent with it being mediated through 5-HT1C receptors. The largest K+-closing responses were induced by a size fraction of mRNA which also induced a large K+ conductance, suggesting that the response requires expression of both receptors and K+ channels. The K+-closing response induced in the oocyte resembles the M- and S-type currents described in, respectively, mammalian and invertebrate neurons. © 1990.