MODULATION OF N-METHYL-D-ASPARTATE RECEPTORS BY HYDROXYL RADICALS IN RAT CORTICAL-NEURONS IN-VITRO

被引:45
作者
AIZENMAN, E
机构
[1] Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, E1456-BST
关键词
N-METHYL-D-ASPARTATE RECEPTOR; REDOX MODULATION; OXIDATION; HYDROXYL RADICALS; XANTHINE; XANTHINE OXIDASE; IRON;
D O I
10.1016/0304-3940(95)11442-Y
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Oxygen-derived reactive species generated by xanthine/xanthine oxidase (X/XO) can modulate the N-methyl-D-aspartate (NMDA) receptor via its redox-sensitive site. Here it is shown that hydroxyl radicals are the agents responsible for NMDA receptor oxidation by X/XO. Spectrophotometric assays revealed that the amounts of superoxide anion and H2O2 produced by X/XO were not decreased by the hydroxyl radical-specific scavenger mannitol. This sugar, however, could prevent most of the oxidizing actions of NMDA receptors by X/XO, but not by the thiol oxidizing agent 5,5'-dithio-bis-nitrobenzoic acid. Finally, a non-enzymatic source of hydroxyl radicals was also effective in oxidizing the receptor's redox site. Hydroxyl radicals may thus represent the final common pathway for the modulation of NMDA receptor function by oxygen-derived free radical generating systems.
引用
收藏
页码:57 / 59
页数:3
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