MECHANISM OF COMPETENCE IN TRANSFORMATION OF STREPTOCOCCI OF SEROLOGICAL GROUP H - PURIFICATION AND SOME PROPERTIES OF COMPETENCE FACTOR

被引：19
作者：
OSOWIECKI, H
NALECZ, J
DOBRZANSKI, WT
机构：
[1] Department of Microbiology and Hygiene, University Medical School, Warsaw
来源：
MOLECULAR AND GENERAL GENETICS | 1969年 / 105卷 / 01期
关键词：
D O I：
10.1007/BF00750310
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In Streptococcus challis, similarly as in the other transforming systems, the appearance of competence (i.e. the ability to take up DNA and undergo transformation) is strictly related to the production by the cells of a special competence factor (CF). As we reported previously, S. challis CF or an essential fragment of its seems to be a polypeptide. In the present work, the method of the purification of S. challis CF on CM-cellulose is described. Highly purified (O.D. at 280 mμ-0,05-0.1) freeze-dried preparations of this factor were obtained. The results of chromatography on Sephadex and Bio-Gels columns suggest, that the mol. w. of the S. challis CF is about 5,000. Highly purified preparations of the CF, besides the competence inducing activity, contain a factor (s) which inactivates transforming DNA in vitro and appears to include some DNase-like activity. © 1969 Springer-Verlag.
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页码：16 / +
页数：1
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