A SPECIES-SPECIFIC DNA-PROBE FOR THE DETECTION OF MYCOPLASMA-AGALACTIAE

被引：15
|
作者：
TOLA, S
RIZZU, P
LEORI, G
机构：
[1] Istituto Zooprofilattico Sperimentale G. Pegreffi
来源：
VETERINARY MICROBIOLOGY | 1994年 / 41卷 / 04期
关键词：
MYCOPLASMA AGALACTIAE; SHEEP; CONTAGIOUS AGALACTIA; DIAGNOSIS; BACTERIA;
D O I：
10.1016/0378-1135(94)90031-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A 4.5 kb M. agalactiae DNA fragment present in strains from different areas of Sardinia was cloned and used to detect M. agalactiae DNA in sheep milk samples by dot blot hybridization. The probe recognized only M. agalactiae strains and did not cross-hybridize with other mycoplasmas (M. capricolum, M. mycoides subsp. capri, M. mycoides subsp. mycoides, M. putrefaciens and M. arginini) or bacteria (E. coli, P. hemolytica, S. aureus, S. epidermis, L. casei, S. durans, S. lactis and S. thermophilus) which may also be found in sheep milk.
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页码：355 / 361
页数：7
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