OXIDATION OF BIPHENYL BY A MULTICOMPONENT ENZYME-SYSTEM FROM PSEUDOMONAS SP STRAIN LB400

被引:59
作者
HADDOCK, JD [1 ]
NADIM, LM [1 ]
GIBSON, DT [1 ]
机构
[1] UNIV IOWA,CTR BIOCATALYSIS & BIOPROC,IOWA CITY,IA 52242
关键词
D O I
10.1128/JB.175.2.395-400.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphenyl. Incorporation of both atoms of molecular oxygen into the substrate was shown with O-18(2). The nonlinear relationship between enzyme activity and protein concentration suggested that the enzyme is composed of multiple protein components. Ion-exchange chromatography of the cell extract gave three protein fractions that were required together to restore enzymatic activity. Similarities with other multicomponent aromatic hydrocarbon dioxygenases indicated that biphenyl dioxygenase may consist of a flavoprotein and iron-sulfur proteins that constitute a short electron transport chain involved in catalyzing the incorporation of both atoms of molecular oxygen into the aromatic ring.
引用
收藏
页码:395 / 400
页数:6
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