VIRULENCE CHARACTERISTICS OF HETEROTROPHIC BACTERIA COMMONLY ISOLATED FROM POTABLE WATER

被引：8
作者：
LYE, DJ
DUFOUR, AP
机构：
[1] Microbiology Research Division, U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, Cincinnati, Ohio, 45268
来源：
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1993年 / 8卷 / 01期
关键词：
D O I：
10.1002/tox.2530080103
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Heterotrophic bacteria isolated from drinking water distribution systems were examined to determine if they possessed putative virulence factors such as hemolysins, proteases, or cytotoxins. Representative samples of colonies from several different distribution systems indicated that the median percentage of heterotrophic bacteria having at least one putative virulence characteristic was about 11% and all of the samples contained bacteria expressing at least one putative virulence characteristic. The overall frequency of bacteria from drinking water distribution samples expressing more than one putative virulence factor ranged from 0 to 8%. The eukaryotic cell culture cytotoxicity test appeared to be the most reliable indicator of those bacteria expressing multiple virulence factors. The results also indicated that the expression of putative virulence factors was highly dependent upon the type of isolation medium used. Although slow-growing heterotrophic bacteria occurred at high densities in certain systems, they expressed very few of the putative virulence factors observed in this study.
引用
收藏
页码：13 / 23
页数：11
相关论文
共 22 条
  • [1] Standard Methods for the Examination of Water and Wastewater, (1985)
  • [2] Asao T., Kinoshita Y., Kozak S., Umera T., Sakaguchi G., Purification and some properties of Aeromonas hydrophila hemolysin, Infect. Imun., 46, pp. 122-127, (1984)
  • [3] Berkelman R.L., Godley J., Weber J.A., Anderson R.L., Lerner A.M., Peterson N.J., Allen J.R., Pseudomonas cepacia peritonitis associated with contamination of automatic peritoneal dialysis machines, Ann. Intern. Med., 96, (1982)
  • [4] Calderon R.L., Levin M.A., Quantitative method for enumeration of enterotoxigenic Escherichia coli, J Clin. Microbiol., 13, pp. 130-134, (1981)
  • [5] Catalogue of Cell Lines and Hybridomas
  • [6] Foley J.F., Gravelle C.R., Englehard W.E., Chin T.D.Y., Achromobacter septicemia‐Fatalities in prematures, American Journal of Diseases of Children, 101, (1961)
  • [7] Holder L.A., Bacterial Enzymes and Virulence, (1985)
  • [8] Keusch G.T., Donta S.T., Classifications of enterotoxins on the basis of activity in cell culture, J. Infect. Dis., 131, pp. 58-63, (1975)
  • [9] Lechevallier M.W., Seidler R.J., Evans T.M., Enumeration and characterization of standard plate count bacteria in chlorinated and raw water samples, Appl. Environ. Microbiol., 40, pp. 922-930, (1980)
  • [10] Ljungh A., Wadstrom T., Toxins of Vibrio parahaemolyticus and Aeromonas hydrophila, J. Toxicol., 1, pp. 257-307, (1983)
123