ISOLATION AND CHARACTERIZATION OF ARGININOSUCCINATE SYNTHETASE FROM HUMAN-LIVER

This communication describes the purification and characterization of argininosuccinate synthetase from human liver. By numerous criteria including electrophoresis in sodium dodecyl sulfate containing gels, electrophoresis in nondissociating gels, and analytical ultracentrifugation, the protein is homogeneous at a specific activity of 4.2 μmol/(min mg) assayed at 37 °C in the direction of argininosuccinate synthesis. The enzyme has a molecular weight of 183 000, as determined by gel filtration. Electrophoresis in the presence of sodium dodecyl sulfate yielded a single band migrating with an Rf corresponding to 43 000 daltons. Thus, the enzyme is considered to contain four subunits of identical molecular weight. The s2o,w of the enzyme is 8.2 S. Antibodies were prepared in rabbits directed against the purified protein. These antibodies react specifically with argininosuccinate synthetase, as determined by electrophoretic analysis of the immunoadsorbed product from crude extracts of human liver. The human enzyme has very similar properties to those published for the beef and rat liver enzymes. © 1979, American Chemical Society. All rights reserved.