RAPID AND PRECISE ASSAY FOR TETRODOTOXIN BINDING TO DETERGENT EXTRACTS OF EXCITABLE TISSUES

被引：46
作者：
LEVINSON, SR
CURATALO, CJ
REED, J
RAFTERY, MA
机构：
[1] Church Laboratory, Chemical Biology Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90045-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tetrodotoxin binding sites in detergent extracts of electric organ membranes from Electrophorus electricus may be conveniently and precisely quantified by a rapid gel filtration assay. This procedure also allows estimation of the equilibrium and kinetic binding parameters describing the interaction of tetrodotoxin with a soluble site. However, the binding of saxitoxin could not be well quantified by this assay, and serves as an example of the limitations of the method as applied to other ligand/receptors systems. © 1979.
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页码：72 / 84
页数：13
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