CLONING, SEQUENCING, AND MUTATIONAL ANALYSIS OF THE HYB-OPERON ENCODING ESCHERICHIA-COLI HYDROGENASE-2

被引：132
作者：
MENON, NK
CHATELUS, CY
DERVARTANIAN, M
WENDT, JC
SHANMUGAM, KT
PECK, HD
PRZYBYLA, AE
机构：
[1] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602
[2] UNIV FLORIDA,DEPT MICROBIOL & CELL SCI,GAINESVILLE,FL 32611
来源：
JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 14期
关键词：
D O I：
10.1128/jb.176.14.4416-4423.1994
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced, They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grow on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.
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页码：4416 / 4423
页数：8
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共 42 条
[1]   PURIFICATION OF THE MEMBRANE-BOUND HYDROGENASE OF ESCHERICHIA-COLI [J].
ADAMS, MWW ;
HALL, DO .
BIOCHEMICAL JOURNAL, 1979, 183 (01) :11-22
[2]   LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].
BACHMANN, BJ .
MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197
[3]   ISOLATION AND CHARACTERIZATION OF A SOLUBLE ACTIVE FRAGMENT OF HYDROGENASE ISOENZYME-2 FROM THE MEMBRANES OF ANAEROBICALLY GROWN ESCHERICHIA-COLI [J].
BALLANTINE, SP ;
BOXER, DH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 156 (02) :277-284
[4]   NICKEL-CONTAINING HYDROGENASE ISOENZYMES FROM ANAEROBICALLY GROWN ESCHERICHIA-COLI K-12 [J].
BALLANTINE, SP ;
BOXER, DH .
JOURNAL OF BACTERIOLOGY, 1985, 163 (02) :454-459
[5]   NUCLEOTIDE-SEQUENCE OF THE DMSABC OPERON ENCODING THE ANAEROBIC DIMETHYLSULFOXIDE REDUCTASE OF ESCHERICHIA-COLI [J].
BILOUS, PT ;
COLE, ST ;
ANDERSON, WF ;
WEINER, JH .
MOLECULAR MICROBIOLOGY, 1988, 2 (06) :785-795
[6]   NITRATE REDUCTASE OF ESCHERICHIA-COLI - COMPLETION OF THE NUCLEOTIDE-SEQUENCE OF THE NAR OPERON AND REASSESSMENT OF THE ROLE OF THE ALPHA-SUBUNIT AND BETA-SUBUNIT IN IRON-BINDING AND ELECTRON-TRANSFER [J].
BLASCO, F ;
IOBBI, C ;
GIORDANO, G ;
CHIPPAUX, M ;
BONNEFOY, V .
MOLECULAR & GENERAL GENETICS, 1989, 218 (02) :249-256
[7]   NUCLEOTIDE-SEQUENCE AND EXPRESSION OF AN OPERON IN ESCHERICHIA-COLI CODING FOR FORMATE HYDROGENYLASE COMPONENTS [J].
BOHM, R ;
SAUTER, M ;
BOCK, A .
MOLECULAR MICROBIOLOGY, 1990, 4 (02) :231-243
[8]   STRUCTURE, FUNCTION AND EVOLUTION OF BACTERIAL FERREDOXINS [J].
BRUSCHI, M ;
GUERLESQUIN, F .
FEMS MICROBIOLOGY LETTERS, 1988, 54 (02) :155-175
[9]   THE HYDROGENASE STRUCTURAL OPERON IN RHODOBACTER-CAPSULATUS CONTAINS A 3RD-GENE, HUPM, NECESSARY FOR THE FORMATION OF A PHYSIOLOGICALLY COMPETENT HYDROGENASE [J].
CAUVIN, B ;
COLBEAU, A ;
VIGNAIS, PM .
MOLECULAR MICROBIOLOGY, 1991, 5 (10) :2519-2527
[10]   2 OPEN READING FRAMES (ORFS) IDENTIFIED NEAR THE HYDROGENASE STRUCTURAL GENES IN AZOTOBACTER-VINELANDII, THE 1ST ORF MAY ENCODE FOR A POLYPEPTIDE SIMILAR TO RUBREDOXINS [J].
CHEN, JC ;
MORTENSON, LE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1131 (01) :122-124
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