A REGULATORY ROLE FOR ARF6 IN RECEPTOR-MEDIATED ENDOCYTOSIS

被引：371

作者：

D'SOUZA-SCHOREY, C ^{[1
]}

LI, GP ^{[1
]}

COLOMBO, MI ^{[1
]}

STAHL, PD ^{[1
]}

机构：

[1] WASHINGTON UNIV, SCH MED, DEPT CELL BIOL & PHYSIOL, ST LOUIS, MO 63110 USA

来源：

SCIENCE | 1995年 / 267卷 / 5201期

关键词：

D O I：

10.1126/science.7855600

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, and the effects on receptor-mediated endocytosis were assessed. Overexpressed ARF6 localized to the cell periphery and led to a redistribution of transferrin receptors to the cell surface and a decrease in the rate of uptake of transferrin. Similar results were obtained when a mutant defective in guanosine triphosphate hydrolysis was expressed. Expression of a dominant negative mutant, ARF6(T27N), resulted in an intracellular distribution of transferrin receptors and an inhibition of transferrin recycling to the cell surface. In contrast, overexpression of ARF1 had little or no effect on these parameters of endocytosis.

引用

页码：1175 / 1178

页数：4

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