CYTOGENETIC ANALYSIS OF HUMAN SOLID TUMORS BY INSITU HYBRIDIZATION WITH A SET OF 12 CHROMOSOME-SPECIFIC DNA PROBES

被引：98
|
作者：
VANDEKKEN, H [1 ]
PIZZOLO, JG [1 ]
REUTER, VE [1 ]
MELAMED, MR [1 ]
机构：
[1] MEM SLOAN KETTERING CANC CTR,DEPT PATHOL,NEW YORK,NY 10021
来源：
CYTOGENETICS AND CELL GENETICS | 1990年 / 54卷 / 3-4期
关键词：
D O I：
10.1159/000132971
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have applied fluorescent in situ hybridization (FISH) to assess the presence of numerical chromosome aberrations in fresh specimens of human solid tumors of varying histology. For this purpose, a set of 12 biotinylated chromosome-specific, repetitive alpha-satellite DNA probes (for chromosomes 1, 6, 7, 9, 10, 11, 15, 16, 17, 18, X, and Y) were hybridized directly to isolated interphase nuclei. Utilizing this approach, we found numerical chromosome changes in all tumors. FISH ploidy profiles were in accordance with flow cytometric DNA histograms of these tumor cells.
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页码：103 / 107
页数：5
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