Genotypes of hepatitis B virus and HbsAg subtypes in Yakutia

The purpose of research. Studying of structure of virus B genotypes and definition of PreS1/PreS2/S-genes heterogeneity in territory of Yakutia. Methods and materials. 16 isolates, allocated in patients with chronic hepatitis B from Ust-Aldanskij region by "nested" PCR method, are studied. Results. The phylogenetic analysis of nucleotide sequences has shown presence of three genotypes of a virus B - A ( 44 %), C ( 12%) and D 944%). Amplification of virus B genome fragments, corresponding PreS1/PreS2/S-genes by "nested" PCR method in blood serums, positive on DNA virus, is lead. The analysis of nucleotide sequences of studied isolates on localization of replacements and deletions in tHis or that site of PreS1/PreS2/S genes has allowed revealing of a significant amount of replacements. The significant genetic variety of viruses of hepatitis B, especially isolates, belonging to VHB A and D genotypes is revealed. Aminoacid replacements causing VHB ability to "diagnostic" escaping are found out. Conclusion. 3 variants of virus B genotype are found out: A, C and D and genetic variety of genotype A and D is revealed.