THE GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON CYTOCHROME B GENE

The purpose of this research was to identify Kejobong goat based on Cytochrome b gene variation by Polymerase Chain Reaction (PCR)-Sequencing technique and to analyze the genetic relationship by using phylogenetic tree. Materials used were blood sample of 12 heads of Kejobong goat from Kaligondang, Pengadegan, Kejobong, and Bukateja Districts. The steps of this research included DNA extraction, PCR, electrophoresis and DNA sequencing. Forward primer 5'-tggaatctaaccatgaccaatg-3' and reverse 3'-ggctattctccttttctggttt-5' were used in PCR process. The results of sequences were then analyzed by ClustalW in the MEGA 5. The result of this study showed that DNA could be extracted well. The good results obtained from sequenced for about 779 bp at the nucleotide of 238 to 1016 in Cytochrome b. A total of 7 codons diverse sites were detected (sites of 103; 122; 165; 167; 198; 215 and 284) at Kejobong goats. There was change in amino acid at 2 sites, those were at 165 sites (*) replaced by (W) in K1 and K2 samples, and at 215 sites (T) replaced by (A) in all samples of Kejobong goats. Kejobong goats at 4 Districts had low genetic distance (0.000-0.001). The analysis of Cytochrome b gene of Kejobong goats originated from different area showed the high similarity and a close genetic relationship.