FURTHER CHARACTERIZATION OF ALLERGENICALLY ACTIVE OLIGOSACCHARITOLS ISOLATED FROM A SEA SQUIRT H-ANTIGEN

被引：38
作者：
OHTA, M
MATSUURA, F
KOBAYASHI, Y
SHIGETA, S
ONO, K
OKA, S
机构：
[1] FUKUYAMA UNIV,FAC ENGN,DEPT BIOTECHNOL,FUKUYAMA,HIROSHIMA 72902,JAPAN
[2] OSAKA UNIV,INST PROTEIN RES,SUITA,OSAKA 564,JAPAN
[3] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIROSHIMA 724,JAPAN
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 290卷 / 02期
关键词：
D O I：
10.1016/0003-9861(91)90569-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Complete primary structures of five allergenically active oligosaccharitols (HPG-β2-N5a, -N6, -N7a, -N7b, and -N9) derived from a sea squirt H-antigen were studied. Structural characterization was carried out by a new method in which products of limited periodate oxidation, followed by derivatization with p-aminobenzoic acid ethyl ester, were analyzed by a combination of HPLC, fast atom-bombardment mass spectrometry, sequential glycosidase digestion, methylation analysis, and 500MHz 1H NMR. Established structures of GalNAcβ14 (GalNAcα1-2Fucα1-3) GlcNAcβ1-3GalNAc-ol, GalNAcβ1-4GlcNAcβ1-3 (GalNAcβ1-4GlcNAcβ1-6) GalNAc-ol, GalNAcβ1-4GlcNAcβ1-3[GalNAcβ1-4 (Fucα1-3) GlcNAcβ1-6] GalNAc-ol, GalNAcβ1-4 (Fucα1-3) GlcNAcβ1-3 [GalNAcβ1-4 (Fucα1-3) GlcNAcβ1-6 ] GalNAc-ol, and GalNAcβ1-4 (GalNAcα1-2Fucα1-3) GlcNAcβ1-3 [GalNAcβ1-4 (GalNAcα1-2Fucα1-3)GlcNAcβ1-6]GalNAc-ol are represented by HPG-β2-N5a, -N6, -N7a, -N7b, and -N9, respectively. These structures have not been encountered previously. Oligosaccharide units GalNAcα1-2 Fucα1-, GalNAcβ1-4GlcNAcβ1-, and Fucα1-3 GlcNAcβ1- are considered to be the allergenically specific epitopes. Partial assignments of 500-MHz 1H NMR spectra of these novel O-linked oligosaccharitols were attempted. © 1991.
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页码：474 / 483
页数：10
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