LITHIUM-CHLORIDE EFFECT ON PHENYLETHYL-B-D-GALACTOSIDE SYNTHESIS BY ASPERGILLUS-ORYZAE B-D-GALACTOSIDASE IN THE PRESENCE OF HIGH LACTOSE CONCENTRATION

被引：15
作者：
FORTUN, Y [1 ]
COLAS, B [1 ]
机构：
[1] FAC SCI & TECH NANTES,CTR RECH BIOL & PHYSICOCHIM CELLULAIRES,BIOCHIM LAB 2,F-44072 NANTES 03,FRANCE
来源：
BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 12期
关键词：
D O I：
10.1007/BF01022087
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Phenylethyl-beta-D-galactoside (PEGal) synthesis by transglycosylation can be catalysed by Aspergillus oryzae beta-galactosidase and was studied using high concentrations of glycosyl donor (lactose). The amount of PEGal formed reached a maximum value after 10h in the presence of 0.5M lactose. Beyond this time, PEGal was slowly hydrolysed. LiCl between 0.4M and 0.7M in the reaction medium prevented the progressive disappearance of the product.
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页码：863 / 866
页数：4
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