RECEPTORS OF THE SEROTONIN-1C SUBTYPE EXPRESSED FROM CLONED DNA MEDIATE THE CLOSING OF K+ MEMBRANE CHANNELS ENCODED BY BRAIN MESSENGER-RNA

被引：28

作者：

PANICKER, MM

PARKER, I

MILEDI, R

机构：

[1] Lab. of Cell./Molecular Neurbiology, Department of Psychobiology, University of California, Irvine

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 06期

关键词：

XENOPUS OOCYTE; POTASSIUM CHANNEL;

D O I：

10.1073/pnas.88.6.2560

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The modulation of K+ channels by serotonin (5-HT) receptors was studied by coinjecting Xenopus oocytes with mRNA transcribed in vitro from a cloned 5-HT 1C subtype (5-HT1C) receptor gene, together with size-fractionated mRNA isolated from rat cerebral cortex that expresses K+ channels. After intracellular loading with EGTA to block Ca2+-dependent chloride currents, these oocytes responded to 5-HT with an inward current associated with a decrease in membrane conductance. Membrane current responses were small or absent in oocytes injected with either mRNA alone. We conclude that 5-HT1C receptors are able to cause the closing of a class of K+ channels expressed by cortex mRNA in a Ca2+-independent manner. The coupling between the receptors and channels appears to be mediated by the inositol phospholipid second messenger pathway, since activation of this pathway by application of serum evoked a similar closing current.

引用

页码：2560 / 2562

页数：3

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