MAP-1 AND MAP-2 BINDING-SITES AT THE C-TERMINUS OF BETA-TUBULIN - STUDIES WITH SYNTHETIC TUBULIN PEPTIDES

被引：72
作者：
CROSS, D
DOMINGUEZ, J
MACCIONI, RB
AVILA, J
机构：
[1] INT CTR CANC & DEV BIOL,CASILLA 70111,SANTIAGO 7,CHILE
[2] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,E-28049 MADRID,SPAIN
来源：
BIOCHEMISTRY | 1991年 / 30卷 / 17期
关键词：
D O I：
10.1021/bi00231a036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The interaction of microtubule-associated proteins MAP-1 and MAP-2 with different peptides containing sequences covering the C-terminal region of beta-tubulin isoforms has been analyzed. Our results indicate that MAP-1 and MAP-2 bind to a common sequence within the variable C-terminal region of the different beta-tubulin isoforms, while MAP-2 also interacts with the subdomain beta(422-434) of the constant region, in agreement with previous results (Maccioni, R. B., Rivas, C., & Vera, J. C. (1988) EMBO J. 7, 1957-1963). The productive interaction of MAP-2 with the latter domain appears to be involved in the assembly of microtubules.
引用
收藏
页码：4362 / 4366
页数：5
相关论文
共 34 条
[1]   ARRANGEMENT OF HIGH MOLECULAR-WEIGHT ASSOCIATED PROTEINS ON PURIFIED MAMMALIAN BRAIN MICROTUBULES [J].
AMOS, LA .
JOURNAL OF CELL BIOLOGY, 1977, 72 (03) :642-654
[2]  
BAUDIER J, 1988, J BIOL CHEM, V263, P5876
[3]  
BHATTACHARYYA B, 1985, J BIOL CHEM, V260
[4]   TUBULIN SITE INTERPRETATION [J].
CLEVELAND, DW ;
JOSHI, HC ;
MURPHY, DB .
NATURE, 1990, 344 (6265) :389-389
[5]   THE TUBULIN-BINDING SEQUENCE OF BRAIN MICROTUBULE-ASSOCIATED PROTEINS, TAU AND MAP-2, IS ALSO INVOLVED IN ACTIN BINDING [J].
CORREAS, I ;
PADILLA, R ;
AVILA, J .
BIOCHEMICAL JOURNAL, 1990, 269 (01) :61-64
[6]  
DIAZNIDO J, 1990, J BIOL CHEM, V265, P13949
[7]   TAU-PROTEIN FUNCTION IN LIVING CELLS [J].
DRUBIN, DG ;
KIRSCHNER, MW .
JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2739-2746
[8]   SELECTIVE PURIFICATION OF MICROTUBULE-ASSOCIATED PROTEIN-1 AND PROTEIN-2 FROM RAT-BRAIN USING POLY(L-ASPARTIC ACID) [J].
FUJII, T ;
NAKAMURA, A ;
OGOMA, Y ;
KONDO, Y ;
ARAI, T .
ANALYTICAL BIOCHEMISTRY, 1990, 184 (02) :268-273
[9]   CLONING AND SEQUENCING OF THE CDNA-ENCODING A CORE PROTEIN OF THE PAIRED HELICAL FILAMENT OF ALZHEIMER-DISEASE - IDENTIFICATION AS THE MICROTUBULE-ASSOCIATED PROTEIN TAU [J].
GOEDERT, M ;
WISCHIK, CM ;
CROWTHER, RA ;
WALKER, JE ;
KLUG, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :4051-4055
[10]   THE PATTERN OF MAP-2 BINDING ON MICROTUBULES - VISUAL ENHANCEMENT OF MAP ATTACHMENT SITES BY ANTIBODY LABELING AND ELECTRON-MICROSCOPY [J].
GOTTLIEB, RA ;
MURPHY, DB .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1983, 85 (02) :175-185
1234