MOLECULAR ANALYSIS OF 2-DIMENSIONAL PROTEIN CRYSTALLIZATION

被引:37
作者
KU, AC
DARST, SA
ROBERTSON, CR
GAST, AP
KORNBERG, RD
机构
[1] STANFORD UNIV,DEPT CELL BIOL,STANFORD,CA 94305
[2] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305
关键词
D O I
10.1021/j100114a030
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Two-dimensional crystals of streptavidin formed on lipid layers at the air-water interface were cross-linked to preserve their structure during transfer to a solid support. The morphology of the crystals viewed at the air-water interface by light microscopy could then be related to the molecular structure revealed in the electron microscope. The preferred growth direction of the crystals proved to be along one of two intersecting rows of intermolecular contacts. A secondary direction arises during a transition to dendritic growth.
引用
收藏
页码:3013 / 3016
页数:4
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