FLUOROMETRIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF FREE FATTY-ACIDS USING PANACYL BROMIDE

被引：6
作者：
STEIN, J
MILOVIC, V
ZEUZEM, S
CASPARY, WF
机构：
[1] Division of Gastroenterology, Department of Internal Medicine, Johann-Wolfgang-Goethe- University, D-6000 Frankfurt 70
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 13期
关键词：
D O I：
10.1080/10826079308019623
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The application of panacyl bromide, a fluorescence labeling reagent for carboxylic acids, was examined. Fatty acids are separated by reversed-phase high-performance liquid chromatography. The derivatives of series of both saturated and unsaturated fatty acids (C10:0-C20:4) are simultaneously separated by a continuous gradient elution method using a methanol-based solvent containing acetonitrile. The quantitative detection is linear over a range of 10-500 pmol per injection. The method was demonstrated to be able to quantify free fatty acids in blood samples from normal subjects and diabetic patients.
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页码：2915 / 2922
页数：8
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