AN ISOTOPIC MICROMETHOD FOR MEASUREMENT OF CHOLINESTERASE ACTIVITY IN INDIVIDUAL CELLS

被引：19
作者：
KOSLOW, SH
GIACOBINI, E
机构：
[1] Department of Pharmacology, Karolinska Institutet, Stockholm
来源：
JOURNAL OF NEUROCHEMISTRY | 1969年 / 16卷 / 11期
关键词：
D O I：
10.1111/j.1471-4159.1969.tb09907.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
—A microisotopic method for measuring acetylcholinesterase activity in isolated cells is described. The assay employs [14C]acetylcholine and can measure 7 × 10‐12 moles of acetylcholine hydrolysed/hr in 50‐150 samples per experiment. The method described has been applied to the measurement of cholinesterase activity in individual sympathetic ganglion cells of the cat. It has been shown that under standard conditions the substrate has complete access to the enzymatic site. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：1523 / +
页数：1
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