EFFECTS OF THE PHOSPHATASE INHIBITOR CALYCULIN-A ON THE PHOSPHORYLATION OF C-PROTEIN IN MAMMALIAN VENTRICULAR CARDIOMYOCYTES

被引：16
|
作者：
NEUMANN, J
BOKNIK, P
KASPAREIT, G
BARTEL, S
KRAUSE, EG
PASK, HT
SCHMITZ, W
SCHOLZ, H
机构：
[1] UNIV MUNSTER, INST PHARMAKOL & TOXIKOL, D-48149 MUNSTER, GERMANY
[2] MAX DELBRUCK CENTRUM MOLEK MED, D-13122 BERLIN, GERMANY
[3] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED, BLACKETT LAB, LONDON SW7 2BZ, ENGLAND
来源：
BIOCHEMICAL PHARMACOLOGY | 1995年 / 49卷 / 11期
关键词：
PROTEIN PHOSPHORYLATION; PHOSPHATASE INHIBITOR; CALYCULIN A; C-PROTEIN;
D O I：
10.1016/0006-2952(95)00101-5
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The effects of inhibitors of protein phosphatase activity on C-protein phosphorylation were studied in preparations from mammalian ventricles. Calyculin A (CyA), an inhibitor of type 1 and 2A protein phosphatases, was studied. CyA concentration- and time-dependency increased the phosphorylation state of C-protein in isolated P-32-labelled guinea pig ventricular cardiomyocytes. C-protein was identified by its reaction with a polyclonal antibody and immunoprecipitation. It is concluded that C-protein in intact cardiomyocytes could be a substrate for type 1 and 2A protein phosphatases.
引用
收藏
页码：1583 / 1588
页数：6
相关论文
共 50 条
  • [1] CALYCULIN-A, PROTEIN PHOSPHATASE INHIBITOR, ENHANCES CAPACITATION OF HUMAN SPERM
    FURUYA, S
    ENDO, Y
    OSUMI, K
    OBA, M
    NOZAWA, S
    SUZUKI, S
    FERTILITY AND STERILITY, 1993, 59 (01) : 216 - 222
  • [2] EFFECTS OF THE PROTEIN PHOSPHATASE INHIBITORS, TAUTOMYCIN AND CALYCULIN-A, ON PROTEIN-PHOSPHORYLATION AND CYTOSKELETON OF HUMAN PLATELETS
    KURISAKI, T
    TAYLOR, RG
    HARTSHORNE, DJ
    CELL STRUCTURE AND FUNCTION, 1995, 20 (05) : 331 - 343
  • [3] BIOCHEMICAL AND ELECTROPHYSIOLOGICAL MECHANISMS OF THE POSITIVE INOTROPIC EFFECT OF CALYCULIN-A, A PROTEIN PHOSPHATASE INHIBITOR
    NEUMANN, J
    BOKNIK, P
    HERZIG, S
    SCHMITZ, W
    SCHOLZ, H
    WIECHEN, K
    ZIMMERMANN, N
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1994, 271 (01): : 535 - 541
  • [4] ENHANCEMENT OF X-RAY CELL-KILLING IN CULTURED-MAMMALIAN-CELLS BY THE PROTEIN PHOSPHATASE INHIBITOR CALYCULIN-A
    NAKAMURA, K
    ANTOKU, S
    CANCER RESEARCH, 1994, 54 (08) : 2088 - 2090
  • [5] DIFFERENTIAL TOXICITY OF THE PROTEIN PHOSPHATASE INHIBITORS MICROCYSTIN AND CALYCULIN-A
    RUNNEGAR, MT
    MADDATU, T
    DELEVE, LD
    BERNDT, N
    GOVINDARAJAN, S
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1995, 273 (01): : 545 - 553
  • [6] THE PROTEIN PHOSPHATASE INHIBITOR CALYCULIN-A STIMULATES CHEMOKINE PRODUCTION BY HUMAN SYNOVIAL-CELLS
    JORDAN, NJ
    WATSON, ML
    WESTWICK, J
    BIOCHEMICAL JOURNAL, 1995, 311 : 89 - 95
  • [7] REGULATION OF MAMMALIAN RIBONUCLEOTIDE REDUCTASE BY THE TUMOR PROMOTERS AND PROTEIN PHOSPHATASE INHIBITORS OKADAIC ACID AND CALYCULIN-A
    HURTA, RAR
    WRIGHT, JA
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1992, 70 (10-11): : 1081 - 1087
  • [8] THE ACTION OF THE PROTEIN-PHOSPHATASE INHIBITOR CALYCULIN-A ON MUSCLE STRIP PREPARATIONS FROM HUMAN MYOCARDIUM
    RUF, T
    HEBISCH, S
    MINALE, C
    MINAMI, K
    HOLUBARSCH, C
    CIRCULATION, 1994, 90 (04) : 174 - 174
  • [9] The myosin phosphatase inhibitor calyculin-A abolishes nitrergic relaxation of the mouse anococcygeus
    Gibson, A
    Ayman, S
    Wallace, P
    McFadzean, I
    BRITISH JOURNAL OF PHARMACOLOGY, 2003, 138 : U59 - U59
  • [10] CALYCULIN-A AND OKADAIC ACID - INHIBITORS OF PROTEIN PHOSPHATASE-ACTIVITY
    ISHIHARA, H
    MARTIN, BL
    BRAUTIGAN, DL
    KARAKI, H
    OZAKI, H
    KATO, Y
    FUSETANI, N
    WATABE, S
    HASHIMOTO, K
    UEMURA, D
    HARTSHORNE, DJ
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (03) : 871 - 877
12345