IDENTIFICATION OF 2 NEW MEMBERS OF THE 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE-ENCODING MULTIGENE FAMILY IN MUNG BEAN

被引：47

作者：

BOTELLA, JR

SCHLAGNHAUFER, CD

ARTECA, JM

ARTECA, RN

PHILLIPS, AT

机构：

[1] PENN STATE UNIV,DEPT HORT,103 TYSON BLDG,UNIV PK,PA 16802

[2] PENN STATE UNIV,DEPT MOLEC & CELLULAR BIOL,UNIV PK,PA 16802

来源：

GENE | 1993年 / 123卷 / 02期

关键词：

ETHYLENE; POLYMERASE CHAIN REACTION; RECOMBINANT DNA; VIGNA-RADIATA;

D O I：

10.1016/0378-1119(93)90132-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The key enzyme regulating ethylene biosynthesis in higher plants is 1-aminocyclopropane-1-carboxylate (ACC) synthase. In mung bean (MB), the existence of three genes encoding this enzyme has previously been reported [Botella et al., Plant Mol. Biol. 18 (1992) 793-797], one of which corresponds to a full-length indole-3-acetic acid-inducible cDNA [Botella et al., Plant Mol. Biol. (1992) 425-436]. In this paper we report the cloning of two new genomic sequences coding for ACC synthase in MB (MAC-4 and MAC-5). MAC-4 is 1340 bp long and encodes 388 amino acids (aa) while MAC-5 is 1393 bp long and encodes for 391 aa. Genomic Southern analysis suggests the existence of only one copy of each gene in the genome.

引用

页码：249 / 253

页数：5

共 11 条

[1]

Abeles F.B, 1973, ETHYLENE PLANT BIOL

[2] IDENTIFICATION AND CHARACTERIZATION OF A FULL-LENGTH CDNA-ENCODING FOR AN AUXIN-INDUCED 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM ETIOLATED MUNG BEAN HYPOCOTYL SEGMENTS AND EXPRESSION OF ITS MESSENGER-RNA IN RESPONSE TO INDOLE-3-ACETIC-ACID [J].