EFFECTS OF INOSITOL TRISPHOSPHATE ON CALCIUM MOBILIZATION IN BONE-CELLS

被引：15

作者：

FALSAFI, R

TATAKIS, DN

HAGELBRADWAY, S

DZIAK, R

机构：

[1] Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, 14214, New York

来源：

CALCIFIED TISSUE INTERNATIONAL | 1991年 / 49卷 / 05期

关键词：

INOSITOL 1,4,5 TRISPHOSPHATE; CALCIUM; OSTEOBLASTS; BONE CELLS;

D O I：

10.1007/BF02556256

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10-mu-M) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP2). Inositol 1,3,4,5 tetrakisphosphate (IP4) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP3 effect. The results are consistent with the hypothesis that increases in IP3 can cause a rapid elevation of bone cell cytosolic calcium.

引用

页码：333 / 339

页数：7

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