Focusing of and imaging with atoms by means of a spherical Fresnel zone plate has been observed for the first time. An intense beam of metastable helium atoms with atomic de Broglie wavelength lambda(DB) = 0.5-2.5 angstrom is passed through either a single or a double slit with dimensions in the 10-mu-m range. This transverse intensity distribution is imaged by a Fresnel zone plate, 210-mu-m in diameter and with an innermost zone diameter of 18.76-mu-m. For lambda(dB) = 1.96 angstrom the focal length is 0.45 m. The imaging properties of the zone plate are presented and compared with numerical calculations.