ABSCISIC ACID-INDUCED ELEVATION OF GUARD-CELL CYTOSOLIC CA-2+ PRECEDES STOMATAL CLOSURE

被引:441
作者
MCAINSH, MR [1 ]
BROWNLEE, C [1 ]
HETHERINGTON, AM [1 ]
机构
[1] MARINE BIOL ASSOC UNITED KINGDOM LAB,PLYMOUTH PL1 2PB,DEVON,ENGLAND
关键词
D O I
10.1038/343186a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
STOMATTA allow the diffusion of CO2 into the leaf for photosynthesis and the diffusion of H2O out of the leaf during transpiration1,2. This gaseous exchange is regulated by pairs of guard cells that surround each stomatal pore. During water stress the loss of water through transpiration is reduced in response to abscisic acid3, a naturally occurring plant growth regulator which is also present in certain mammals4, algae5 and fungi6, by the promotion of stomatal closure and inhibition of opening7. This involves alterations to guard cell turgor, causing the cells to shrink and thereby reducing the size of the stomatal pore. These changes are driven by cation and anion effluxes8. It has been proposed that an abscisic acid-dependent increase in the concentration of guard cell cytosolic free calcium triggers the intracellular machinery responsible for stomatal closure9(for a review, see ref. 10), but attempts to test this hypothesis by measuring [45Ca] fluxes have produced equivocal results11. Using the fluorescent calcium indicator fura-2, we report that abscisic acid induces a rapid increase in guard cell cytosolic free Ca2+ in Commelina communisL., and that this increase precedes stomatal closure. These results strongly support the sugá-gestion that Ca2+ is an intracellular second messenger in this response. © 1990 Nature Publishing Group.
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页码:186 / 188
页数:3
相关论文
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