VALIDATION OF A HPLC METHOD FOR OCHRATOXIN A DETERMINATION

Ochratoxin A is a mycotoxin produced by various species of Aspergillus and Penicillium. Ochratoxin A has been detected in cereals and cereal products, coffee beans, beer, wine, spices, pig.s kidney and cow.s milk. For ochratoxin A, a HPLC method was developed and validated. Ochratoxin A was determined by RP-HPLC, using a liquid chromatograph type HP 1090 Series II, equiped with a fluorescence detector. The analysis was performed with a Phenomenex column, type Luna C18(2) 100A (150 x 4,6 mm; 5 mu m) with a mobile phase consisting of a mixture of acetonitrile/water/acid acetic (99/99/2), a flow of 0,7 mL/min. For detection, the wavelenght of excitation was 228 nm and wavelenght of emision was 423 nm. The calibration graph was linear in 6,25-50 ng/mL concentration range (r(2) = 0,9991). The detection limits was 1,6 ng/mL and the quantification limit was 4,9 ng/mL. The method precision (RSD = 2,4975 %) and the accuracy (recovery was 100,1 %) were studied. The HPLC method was applyed for ochratoxin A from food samples with good results.