ACTIVATION OF PHOSPHOLIPASE A(2) BY THE HUMAN ENDOTHELIN RECEPTOR IN CHINESE-HAMSTER OVARY CELLS INVOLVES G(I) PROTEIN-MEDIATED CALCIUM INFLUX

被引：16
作者：
KRUGER, H
CARR, S
BRENNAND, JC
MCLEAN, JS
机构：
[1] UNIV PAISLEY,DEPT BIOL SCI,PAISLEY PA1 2BE,RENFREW,SCOTLAND
[2] ZENECA PHARMACEUT,CARDIOVASC & METAB RES DEPT,MACCLESFIELD SK10 4TG,CHESHIRE,ENGLAND
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.2744
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The signalling pathways used by the human endothelin A receptor to activate phospholipase A(2) in Chinese hamster ovary cells were investigated. Pertussis toxin caused a partial but significant reduction in endothelin-1-induced arachidonic acid release although cAMP-dependent kinase inhibitors did not mimic its action. Extracellular calcium and its entry into the cell was essential for activation of phospholipase A(2) as its removal from media or incubation with an intracellular calcium chelator-reduced activation. Nifedipine had no effect on endothelin-1-induced arachidonic acid release while divalent cations caused a significant reduction indicating the possible role of CRAC. Thapsigargin caused an increase in arachidonic acid release which was completely inhibited by pertussis toxin treatment. This further supports the involvement of CRAC in calcium influx and activation of phospholipase A(2) by the human endothelin A receptor. (C) 1995 Academic Press, Inc.
引用
收藏
页码：52 / 58
页数：7
相关论文
共 50 条
[11]   DIFFERENTIAL REGULATION OF C-FOS GENE-EXPRESSION BY 2 TYPES OF HUMAN ENDOTHELIN RECEPTOR IN CHINESE-HAMSTER OVARY CELLS [J].
TABUCHI, H ;
FURUICHI, Y ;
MIYAMOTO, C .
JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1994, 12 (02) :173-180
[12]   CHARACTERIZATION OF THE SUBSTANCE-P RECEPTOR-MEDIATED CALCIUM INFLUX IN CDNA TRANSFECTED CHINESE-HAMSTER OVARY CELLS - A POSSIBLE ROLE OF INOSITOL 1,4,5-TRISPHOSPHATE IN CALCIUM INFLUX [J].
MOCHIZUKIODA, N ;
NAKAJIMA, Y ;
NAKANISHI, S ;
ITO, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (13) :9651-9658
[13]   RECEPTOR-MEDIATED ENDOCYTOSIS OF PLANT AND BACTERIAL TOXINS IN CHINESE-HAMSTER OVARY CELLS [J].
WU, HC ;
GHOSH, PC .
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1988, 25 (1-2) :70-75
[14]   MUTANT CHINESE-HAMSTER OVARY CELLS PLEIOTROPICALLY DEFECTIVE IN RECEPTOR-MEDIATED ENDOCYTOSIS [J].
ROBBINS, AR ;
PENG, SS ;
MARSHALL, JL .
JOURNAL OF CELL BIOLOGY, 1983, 96 (04) :1064-1071
[15]   PHOSPHOLIPASE-C ACTIVATION BY NEUROTENSIN AND NEUROMEDIN-N IN CHINESE-HAMSTER OVARY CELLS EXPRESSING THE RAT NEUROTENSIN RECEPTOR [J].
HERMANS, E ;
MALOTEAUX, JM ;
OCTAVE, JN .
MOLECULAR BRAIN RESEARCH, 1992, 15 (3-4) :332-338
[16]   THE GUANINE-NUCLEOTIDE-BINDING PROTEIN SUBUNIT G-ALPHA(I2) IS INVOLVED IN CALCIUM ACTIVATION OF PHOSPHOLIPASE A(2) - EFFECTS OF THE DOMINANT-NEGATIVE G-ALPHA(I2) MUTANT, [G203T]G-ALPHA(I2), ON ACTIVATION OF PHOSPHOLIPASE A(2) IN CHINESE-HAMSTER OVARY CELLS [J].
MURRAYWHELAN, R ;
REID, JD ;
PIUZ, I ;
HEZAREH, M ;
SCHLEGEL, W .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 230 (01) :164-169
[17]   CALCIUM, ASPARAGINE AND CAMP ARE REQUIRED FOR ORNITHINE DECARBOXYLASE ACTIVATION IN INTACT CHINESE-HAMSTER OVARY CELLS [J].
COSTA, M ;
NYE, JS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 85 (03) :1156-1164
[18]   Barium influx mediated by the cardiac sodium-calcium exchanger in transfected Chinese hamster ovary cells [J].
Condrescu, M ;
Chernaya, G ;
Kalaria, V ;
Reeves, JP .
JOURNAL OF GENERAL PHYSIOLOGY, 1997, 109 (01) :41-51
[19]   ALPHA-2-ADRENERGIC RECEPTOR STIMULATION OF PHOSPHOLIPASE-A2 AND OF ADENYLATE-CYCLASE IN TRANSFECTED CHINESE-HAMSTER OVARY CELLS IS MEDIATED BY DIFFERENT MECHANISMS [J].
JONES, SB ;
HALENDA, SP ;
BYLUND, DB .
MOLECULAR PHARMACOLOGY, 1991, 39 (02) :239-245
[20]   SYNTHESIS OF FUNCTIONAL HUMAN LEUSERPIN-2 IN CHINESE-HAMSTER OVARY CELLS [J].
RAGG, H ;
ULSHOFER, T ;
STEPHAN, M .
THROMBOSIS AND HAEMOSTASIS, 1989, 62 (01) :387-387
12345