ISOLATION AND PURIFICATION OF DIGALACTOSYLDIACYLGLYCEROLS

被引：11

作者：

BERGQVIST, MHJ

HERSLOF, BG

机构：

[1] UNIV STOCKHOLM,DEPT ANALYT CHEM,STOCKHOLM,SWEDEN

[2] KARLSHAMNS LIPIDTEKN AB,S-11384 STOCKHOLM,SWEDEN

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; DIGALACTOSYLDIACYLGLYCEROLS; SOLID PHASE EXTRACTION; OATS; EXPERIMENTAL DESIGN;

D O I：

10.1007/BF02272159

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient method for digalactosyldiacylglycerols from been developed using solid phase extraction. Commercial cartridges were utilized to prepare 100 mg fractions of galactolipids containing more than 97 % of digalactosyldiacylglycerol. The purity of the fractions was monitored by an isocratic normal phase analytical HPLC procedure, which was optimized using factorial design. The optimized system was successfully scaled up to a semi-preparative HPLC system with a capacity of about 10 mg/injection which was used to further purify the digalactosyldiacylglycerol fraction.

引用

页码：129 / 133

页数：5

共 36 条

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