EVIDENCE FOR REDOX FORMS OF THE AEQUOREA GREEN FLUORESCENT PROTEIN

被引：123

作者：

INOUYE, S ^{[1
]}

TSUJI, FI ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV MARINE BIOL RES 0202,LA JOLLA,CA 92093

来源：

FEBS LETTERS | 1994年 / 351卷 / 02期

基金：

美国国家科学基金会;

关键词：

OXIDATION-REDUCTION; ELECTRON TRANSFER; PROTEIN CHROMOPHORE; ENERGY TRANSFER; BIOLUMINESCENCE; AEQUORIN;

D O I：

10.1016/0014-5793(94)00859-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly purified recombinant Aequorea green fluorescent protein is able to undergo a reversible oxidation-reduction reaction in the presence of molecular oxygen. In the oxidized form in near UV light, the protein is highly fluorescent, but when reduced with sodium dithionite, it becomes completely non-fluorescent. On exposure to molecular oxygen the reduced, non-fluorescent protein reverts to its original fluorescent state.

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收藏

页码：211 / 214

页数：4

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