POSTFILTRATION FACTOR-VIII AND FIBRINOGEN LEVELS IN CRYOPRECIPITATE STORED AT ROOM-TEMPERATURE AND AT 1-DEGREES-C TO 6-DEGREES-C

The 13th edition of the standards of the American Association of Blood Banks specified storage at 1 to 6-degrees-C for cryoprecipitated anti-hemophilic factor (Cryo) administered up to 6 hours after thawing if the Cryo is used for factor VIII (FVIII) content (Standard J4.210). Previous editions specified room-temperature (RT) storage for up to 6 hours. Currently, the temperature specification has been deleted. There are few data addressing the optimal storage temperature and maximum storage time for FVIII and fibrinogen in thawed Cryo. Thirty bags of Cryo were assayed for FVIII and fibrinogen. Each bag was divided into two aliquots; one was stored at RT and the other at 1 to 6-degrees-C. Assays were performed immediately after thawing (Base) and 6 and 24 hours after thawing, respectively. All samples were filtered-through 200-mu blood component infusion sets before assay. Three hundred analyses were performed, 150 each for FVIII and fibrinogen by conventional clotting technique. Data were analyzed by using a paired t test. Cryo stored at 1 to 6-degrees-C for 6 and 24 hours showed an FVIII loss of 35 percent (p < 0.0001) and 63 percent (p < 0.0001), respectively. Cryo stored at RT for 6 and 24 hours had an FVIII loss of 8 percent (p > 0.05) and 20 percent (p < 0.0001). Cryo stored at 1 to 6-degrees-C for 6 and 24 hours had a fibrinogen loss of 20 percent (p < 0.0001) and 43 percent (p < 0.0001). Cryo stored at RT for 6 hours had no fibrinogen loss and a 2 percent loss at 24 hours (p > 0.05). These preliminary data show a significant loss of FVIII and fibrinogen activity in Cryo stored at 1 to 6-degrees-C and filtered before assay. The FVIII and fibrinogen activity at RT is clearly maintained up to 6 hours after thawing.