CHANGES IN THE PROTEIN-SYNTHESIS PATTERN DURING A NUTRITIONAL SHIFT-DOWN TRANSITION IN SACCHAROMYCES-CEREVISIAE

被引：2

作者：

RODRIGUEZ, F ^{[1
]}

POPOLO, L ^{[1
]}

VAI, M ^{[1
]}

LACANA, E ^{[1
]}

ALBERGHINA, L ^{[1
]}

机构：

[1] UNIV MILAN,DIPARTIMENTO FISIOL & BIOCHIM GEN,SEZ BIOCHIM COMPARATA,I-20133 MILAN,ITALY

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 187卷 / 02期

关键词：

D O I：

10.1016/0014-4827(90)90097-T

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition. © 1990.

引用

页码：315 / 319

页数：5