SIMPLE METHOD TO PURIFY CHICKEN IMMUNOGLOBULIN-G

被引：19

作者：

BHANUSHALI, JK ^{[1
]}

GILBERT, JM ^{[1
]}

MCDOUGALD, LR ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT POULTRY SCI,ATHENS,GA 30602

来源：

POULTRY SCIENCE | 1994年 / 73卷 / 07期

关键词：

ANTIBODIES; CHICKEN; IMMUNOGLOBULIN-G; CAPRYLIC ACID; PURIFICATION TECHNIQUE;

D O I：

10.3382/ps.0731158

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A new method for purifying IgG was adapted for use with chicken serum. Chicken serum was mixed with caprylic acid, precipitated with ammonium sulfate, and dialyzed against PBS. Analysis by SDS-PAGE and Western blot confirmed successful purification of chicken IgG. Large quantities of highly purified IgG were easily obtainable for use in immunological investigations or for labeling. This technique is rapid, inexpensive, and simpler to perform than traditional ion exchange or gel filtration chromatography.

引用

页码：1158 / 1161

页数：4

共 4 条

[1]

BENEDICT A, 1967, METHODS IMMUNOLOGY I, V1, P119

[2] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].