JUVENILE-HORMONE CATABOLISM IN MANDUCA-SEXTA - HOMOLOG SELECTIVITY OF CATABOLISM AND IDENTIFICATION OF A DIOL-PHOSPHATE CONJUGATE AS A MAJOR END-PRODUCT

被引：45

作者：

HALARNKAR, PP ^{[1
]}

JACKSON, GP ^{[1
]}

STRAUB, KM ^{[1
]}

SCHOOLEY, DA ^{[1
]}

机构：

[1] SYNTEX INC, PALO ALTO, CA 94304 USA

来源：

EXPERIENTIA | 1993年 / 49卷 / 11期

关键词：

JUVENILE HORMONE ESTERASE; JUVENILE HORMONE EPOXIDE HYDROLASE; JUVENILE HORMONE BINDING PROTEIN; REVERSED-PHASE LIQUID CHROMATOGRAPHY ASSAY;

D O I：

10.1007/BF02125646

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We studied time-dependent metabolism of (10R)-[H-3] juvenile hormone (JH) III and (10R, 11S)-[H-3 JH I injected into Manduca sexta larvae; the hormones are metabolized to polar metabolites, especially the JH acid-diol, and an unknown. Products were analyzed using a reversed-phase liquid chromatography assay. (10R)-JH III is metabolized much more rapidly than (10R, 11S)-[H-3]JH 1, whether injected seperately or as a mixture of hormones. The unknown metabolites of JH I and JH III were identified as phosphate conjugates of JH I and JH III diol by tandem mass spectral analysis of isolated samples: The phosphate conjugate of JH I diol is the principle end product of JH I metabolism.

引用

页码：988 / 994

页数：7

共 46 条

[1] COMPARATIVE METABOLISM OF CECROPIA JUVENILE-HORMONE [J].