EXPRESSION OF ALPHA-TROPOMYOSIN DURING CARDIAC DEVELOPMENT IN THE CHICK-EMBRYO

被引：6

作者：

PRADOS, J ^{[1
]}

FERNANDEZ, JE ^{[1
]}

GARRIDO, F ^{[1
]}

ALVAREZ, L ^{[1
]}

HIDALGO-ALVAREZ, R ^{[1
]}

MUROS, MA ^{[1
]}

ARANEGA, A ^{[1
]}

机构：

[1] UNIV GRANADA, SCH MED, DEPT MORPHOL SCI, BASIC CARDIOVASC RES SECT, E-18071 GRANADA, SPAIN

来源：

ANATOMICAL RECORD | 1992年 / 234卷 / 03期

关键词：

CARDIAC ALPHA-TROPOMYOSIN; MONOCLONAL ANTIBODY; CYTOSKELETON; CHICK EMBRYO;

D O I：

10.1002/ar.1092340302

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

A new monoclonal antibody (mAb) that recognizes alpha-tropomyosin in cardiac muscle cells was used in a qualitative (polyacrylamide gel electrophoresis and indirect immunofluorescence) and quantitative (fluorescence-activated cell sorting) study of the expression of this protein during heart development. Alpha-Tropomyosin expression was weak in early stages of chick embryo development (Hamburger and Hamilton stage 18), and increased steadily until Hamburger Hamilton stage 40. In early stages, the protein was found mainly in cytoplasm, whereas by the final stages, it was more abundant in the cytoskeletal compartment. The mAb cross-reacted with alpha-tropomyosin in smooth and striated muscle cells from chickens, mice, and humans, but did not cross-react with nonmuscle tropomyosin.

引用

页码：301 / 309

页数：9

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