EFFECT OF PH ON THE OXYGEN KINETICS OF CYTOCHROME-C OXIDASE PROTEOLIPOSOMES

被引：4
作者：
PETERSEN, LC [1 ]
机构：
[1] ODENSE UNIV,INST BIOCHEM,DK-5230 ODENSE M,DENMARK
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 548卷 / 03期
关键词：
Cytochrome c oxidase; Oxygen kinetics; Proteoliposome;
D O I：
10.1016/0005-2728(79)90070-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of pH on the oxygen kinetics of cytochrome c oxidase incorporated into phospholipid vesicles is studied. The pH profiles of the oxygen kinetics of energized and deenergized oxidase vesicles are similar. An effect of pH on the slope of the reciprocal plot of rate against oxygen concentration is observed, and this may indicate that protons are involved in the rate limiting step of the reaction between oxygen and reduced oxidase. In contrast to the pH dependence of the oxygen kinetics, the binding of CO to the oxidase is not pH dependent. © 1979.
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页码：636 / 641
页数：6
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